This study examined the effect of exercise mode and intensity on physiological and perceptual responses to exercise. Twelve active adults (6 men and 6 women, age = 21.7 ± 1.6 years) initially performed incremental testing on the treadmill (TM) and cycle ergometer (CE) to assess maximal oxygen uptake (V̇o2max) and ventilatory threshold (VT). During the next 4 visits in a randomized order, subjects performed 20 minutes of moderate-intensity continuous exercise (MICE) at an intensity 20% below VT on the TM (TMMICE) and cycle ergometer (CEMICE) as well as time-matched high-intensity interval exercise (HIIE; 10 1-minute bouts at workload equal to 20% above VT followed by 1-minute active recovery) on both modes (TMHIIE and CEHIIE). During exercise, gas exchange data, blood lactate concentration, and perceptual responses (rating of perceived exertion, affective valence, and enjoyment) were assessed. Heart rate (p < 0.001) and V̇o2 (p < 0.001) were higher in response to TMHIIE vs. CEHIIE as well as TMMICE vs. CEMICE. Blood lactate concentration was higher (p = 0.003) in response to CEHIIE vs. TMHIIE. The rating of perceived exertion was significantly higher (p < 0.001) in TMMICEcompared with CEMICE which showed the most positive affective valence (p = 0.009). Enjoyment was similar across all bouts (p = 0.11). Treadmill-based HIIE leads to higher heart rate and V̇o2 vs. CEHIIE, although there was no difference in affective valence or enjoyment. Practitioners aiming to optimize the cardiorespiratory response to moderate or interval exercise in their clientele should recommend TM running rather than cycling.
